Name: Biotinylated Human ACE2/ACEH Protein (ACE-HM401B)
Brand: KactusBio
SKU: ACE-HM401B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve showing interaction between biotinylated human ACE2 and SARS-CoV-2 S1 His-tag protein.

Biotinylated Human ACE2/ACEH Protein (ACE-HM401B)

Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 40.6ng/ml determined by ELISA (QC Test).

Biotinylated Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded SARS-COV-2 Spike S1, hFc Tag on ProA-Biosensor can bind Biotinylated Human ACE2 with an affinity constant of 2.01 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated human ACE2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 40.6ng/ml determined by ELISA (QC Test).
Amino Acid Range: Gln18-Ser740

Catalog #ACE-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human ACE2/ACEH Protein (ACE-HM401B) Summary

Product Description	Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.
Product Background	ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human ACE2/ACEH Protein (ACE-HM401B) Specifications/Details

Protein	ACE2/ACEH
Synonyms	ACE-2; ACEH; ACE2
Accession	Q9BYF1-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gln18-Ser740
Molecular Weight	The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 40.6ng/ml determined by ELISA (QC Test).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 40.6ng/ml determined by ELISA (QC Test).
	Biotinylated Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded SARS-COV-2 Spike S1, hFc Tag on ProA-Biosensor can bind Biotinylated Human ACE2 with an affinity constant of 2.01 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated human ACE2 is greater than 95% as determined by SEC-HPLC.