Biotinylated Human ACE2/ACEH Protein (ACE-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln18-Ser740

Catalog: ACE-HM401B

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Product Description Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.
Product Category Recombinant Protein
Target ACE2/ACEH
Synonyms ACE-2; ACEH; ACE2
Gene/Protein ID Q9BYF1-1
Species Human
Exact Sequence Gln18-Ser740
Molecular Weight The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result.
Activity Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 0.11ug/ml determined by ELISA.
The purity of Biotinylated human ACE2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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