Biotinylated Human ACE2/ACEH Protein (ACE-HM401B)
Biotinylated Human ACE2/ACEH Protein (ACE-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln18-Ser740
Catalog #ACE-HM401B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln18-Ser740.

Product Background

ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.

Product Category

Recombinant Protein / Antibody discovery

Protein

ACE2/ACEH

Synonyms

ACE-2; ACEH; ACE2

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gln18-Ser740

Molecular Weight

The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Bis-Tris PAGE result.

Activity

Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 0.11ug/ml determined by ELISA. Immobilized SARS-COV-2 Spike RBD, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 0.11ug/ml determined by ELISA.
Biotinylated Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated human ACE2 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated human ACE2 is greater than 95% as determined by SEC-HPLC.

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