Biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human ICOS (C136S,C137S) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 23.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp19-Ser258

Catalog: BH7-HM472B

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Product Description Recombinant Biotinylated Human B7-H2/ICOSLG Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Asp19-Ser258.
Product Category Recombinant Protein
Target B7-H2/ICOSLG
Synonyms CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Gene/Protein ID O75144
Species Human
Exact Sequence Asp19-Ser258
Molecular Weight The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human ICOS (C136S,C137S) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 23.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ICOS (C136S,C137S) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 23.9ng/ml determined by ELISA.
The purity of Biotinylated Human B7-H2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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