Name: Biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472B)
Brand: KactusBio
SKU: BH7-HM472B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472B)

Immobilized Human ICOS (C136S,C137S), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 25.1ng/ml determined by ELISA.

Biotinylated Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human B7-H2 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human ICOS (C136S,C137S), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 25.1ng/ml determined by ELISA.
Amino Acid Range: Asp19-Ser258

Catalog #BH7-HM472B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472B) Summary

Product Description	Recombinant Biotinylated Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258.
Product Background	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472B) Specifications/Details

Protein	B7-H2/ICOSLG
Synonyms	CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Accession	O75144
Species	Human
Biotinylated	yes
Amino Acid Range	Asp19-Ser258
Molecular Weight	The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ICOS (C136S,C137S), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 25.1ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human ICOS (C136S,C137S), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 25.1ng/ml determined by ELISA.
	Biotinylated Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human B7-H2 is greater than 90% as determined by SEC-HPLC.