Name: Biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472B)
Brand: KactusBio
SKU: BH7-HM472B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472B)

Immobilized Human ICOS (C136S,C137S), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 25.1ng/ml determined by ELISA.

Biotinylated Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human ICOS (C136S,C137S), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 25.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp19-Ser258

Catalog #BH7-HM472B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258.
Product Background	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	B7-H2/ICOSLG
Synonyms	CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Accession	O75144
Species	Human
Biotinylated	yes
Amino Acid Range	Asp19-Ser258
Molecular Weight	The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ICOS (C136S,C137S), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 25.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human ICOS (C136S,C137S), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 25.1ng/ml determined by ELISA.
	Biotinylated Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.