Biotinylated Human B7-H2/ICOSLG Protein (BH7-HM472B)
Catalog: BH7-HM472B
Order now get it on
| Product Description | Recombinant Biotinylated Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258. |
Product Background |
B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. |
Product Category |
Recombinant Protein |
Target |
B7-H2/ICOSLG |
| Synonyms | CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50 |
Gene/Protein ID |
O75144 |
Species |
Human |
Exact Sequence |
Asp19-Ser258 |
| Molecular Weight | The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human ICOS (C136S,C137S) , His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 23.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human ICOS (C136S,C137S) , His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 23.9ng/ml determined by ELISA. |
|
|
The purity of Biotinylated Human B7-H2 is greater than 95% as determined by SEC-HPLC. |
|
|
Biotinylated Human B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
You may also be interested in
View more associated products