Name: Biotinylated Human CDCP1 Protein (CDC-HM401B)
Brand: KactusBio
SKU: CDC-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human CDCP1 Protein (CDC-HM401B)

Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.

Biotinylated Human CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Biotinylated Human CDCP1, His-Avi Tag with an affinity constant of 3.97 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Human CDCP1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.
Amino Acid Range: Phe30-Leu666

Catalog #CDC-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CDCP1 Protein (CDC-HM401B) Summary

Product Description	Recombinant Biotinylated Human CDCP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe30-Leu666.
Product Background	Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human CDCP1 Protein (CDC-HM401B) Specifications/Details

Protein	CDCP1
Synonyms	CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
Accession	Q9H5V8-1
Species	Human
Biotinylated	yes
Amino Acid Range	Phe30-Leu666
Molecular Weight	The protein has a predicted MW of 74.6 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 100mM Arginine, 8% trehalose (pH 7.4).
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.
	Biotinylated Human CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Biotinylated Human CDCP1, His-Avi Tag with an affinity constant of 3.97 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Human CDCP1 is greater than 95% as determined by SEC-HPLC.