Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein (FCR-HM42AB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: The affinity constant of 1.03 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala36-Ile218

Catalog: FCR-HM42AB

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Product Description Recombinant Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala36-Ile218.
Product Category Recombinant Protein
Target Fc gamma RIIA/CD32a (R167)
Synonyms CD32MGC23887; CDw32fc-gamma-RIIa; Fc gamma RIIA; FCG2; Fc-gamma-RIIa; FcGR; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; IGFR2MGC30032; IgG Fc receptor II-a; FCG2; CD32A
Gene/Protein ID P12318-1
Species Human
Exact Sequence Ala36-Ile218
Molecular Weight The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 1.03 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (R167) , His-Avi Tag with an affinity constant of 1.03 μM as determined in SPR assay (Biacore T200).
The purity of Biotinylated Fc gamma RIIA/CD32a (R167) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIA/CD32a (R167) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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