Name: Biotinylated Human FGF-7/KGF Protein (Primary Amine Labeling) (KGF-HE101B)
Brand: KactusBio
SKU: KGF-HE101B-100UG
Price: 600.0 USD
Availability: InStock

Biotinylated Human FGF-7/KGF Protein (Primary Amine Labeling) (KGF-HE101B)

Human FGFR2 beta (IIIb), hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human FGF-7, His Tag with an affinity constant of 0.74 nM as determined in SPR assay (Biacore T200).

Immobilized Human FGFR2 beta (IIIb), hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human FGF-7, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test).

Biotinylated Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human FGFR2 beta (IIIb), hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human FGF-7, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). The affinity constant of 0.74 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Cys32-Thr194

Catalog #KGF-HE101B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human FGF-7/KGF Protein (Primary Amine Labeling) is expressed from E.coli with His tag at the N-Terminus.It contains Cys32-Thr194.
Product Background	The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.
Product Category	Recombinant Protein / Antibody discovery

Protein	FGF-7/KGF
Synonyms	FGF-7; HBGF-7; KGF
Accession	P21781-1
Species	Human
Biotinylated	yes
Amino Acid Range	Cys32-Thr194
Molecular Weight	The protein has a predicted MW of 20.1 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human FGFR2 beta (IIIb), hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human FGF-7, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test). The affinity constant of 0.74 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 50mM Tris, 250mM NaCl (pH 7.5).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human FGFR2 beta (IIIb), hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human FGF-7, His Tag with an affinity constant of 0.74 nM as determined in SPR assay (Biacore T200).
	Immobilized Human FGFR2 beta (IIIb), hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human FGF-7, His Tag with the EC50 of 0.14ug/ml determined by ELISA (QC Test).
	Biotinylated Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.