Name: Biotinylated Human GFRAL/GFR alpha-like Protein (GFL-HM401B)
Brand: KactusBio
SKU: GFL-HM401B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human GFRAL/GFR alpha-like Protein (GFL-HM401B)

Immobilized Human GDF15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 55.6ng/ml determined by ELISA (QC Test).

Biotinylated Human GFRAL on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.

The purity of-Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GDF15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 55.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ser19-Glu351

Catalog #GFL-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser19-Glu351.
Product Background	GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Product Category	Recombinant Protein / Antibody discovery

Protein	GFRAL/GFR alpha-like
Synonyms	GFR alpha-like; GFRAL; GRAL; C6orf144
Accession	Q6UXV0
Species	Human
Biotinylated	yes
Amino Acid Range	Ser19-Glu351
Molecular Weight	The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GDF15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 55.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human GDF15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 55.6ng/ml determined by ELISA (QC Test).
	Biotinylated Human GFRAL on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.
	The purity of-Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC.