Biotinylated Human GFRAL/GFR alpha-like Protein (GFL-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human GDF15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 77.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser19-Glu351

Catalog: GFL-HM401B

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Product Description Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ser19-Glu351.
Product Category Recombinant Protein
Target GFRAL/GFR alpha-like
Synonyms GFR alpha-like; GFRAL; GRAL; C6orf144
Gene/Protein ID Q6UXV0
Species Human
Exact Sequence Ser19-Glu351
Molecular Weight The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GDF15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 77.6ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GDF15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 77.6ng/ml determined by ELISA.
Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.
The purity of Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GFRAL on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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