Product Description |
Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser19-Glu351. |
Product Background |
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
GFRAL/GFR alpha-like |
Synonyms |
GFR alpha-like; GFRAL; GRAL; C6orf144 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser19-Glu351 |
Molecular Weight |
The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GDF15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 67.1ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human GDF15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 67.1ng/ml determined by ELISA (QC Test). |
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Biotinylated Human GFRAL on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml. |
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The purity of-Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC. |
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