Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer Protein (MHC-HE034B)
Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer Protein (MHC-HE034B)
  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 18.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and EAAGIGILTV peptide
Catalog #MHC-HE034B
Regular price $341.00
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Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer Protein (MHC-HE034B) Summary

Product Description

Recombinant Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and EAAGIGILTV peptide.

Product Background

Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Specifications

Protein
HLA-A*02:01&B2M&MART-1 (EAAGIGILTV)
Synonyms
MART1; LB39-AA; SK29-AA; Melan-A; MLANA

MHC Form

Monomer

Allele

HLA-A*02:01
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and EAAGIGILTV peptide
Molecular Weight
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 18.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 18.1ng/ml determined by ELISA. Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer, His-Avi Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 18.1ng/ml determined by ELISA.
Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 (EAAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.
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