Name: Biotinylated Human IL-10 R alpha Protein (IL1-HM4RAB)
Brand: KactusBio
SKU: IL1-HM4RAB-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human IL-10 R alpha Protein (IL1-HM4RAB)

Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 60.7ng/ml determined by ELISA.

Biotinylated Human IL-10 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human IL-10 R alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 60.7ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: His22-Asn235

Catalog #IL1-HM4RAB

Size

100UG

500UG

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Biotinylated Human IL-10 R alpha Protein (IL1-HM4RAB) Summary

Product Description	Recombinant Biotinylated Human IL-10 R alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His22-Asn235.
Product Background	The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human IL-10 R alpha Protein (IL1-HM4RAB) Specifications/Details

Protein	IL-10R alpha
Synonyms	IL-10RA; IL10RA; IL10R; IL-10R1; IL-10R subunit 1; CDw210a; HIL-10R; IBD28; IL-10 R alpha
Accession	Q13651
Species	Human
Biotinylated	yes
Amino Acid Range	His22-Asn235
Molecular Weight	The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 42-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 60.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 60.7ng/ml determined by ELISA.
	Biotinylated Human IL-10 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human IL-10 R alpha is greater than 95% as determined by SEC-HPLC.