Name: Biotinylated Human IL-17A/CTLA-8 Protein (ILA-HM418B)
Brand: KactusBio
SKU: ILA-HM418B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human IL-17A/CTLA-8 Protein (ILA-HM418B)

Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi Tag with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA (QC Test).

Biotinylated Human IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA (QC Test). The affinity constant of 2.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly24-Ala155

Catalog #ILA-HM418B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Ala155.
Product Background	Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-17A/CTLA-8
Synonyms	CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A
Accession	Q16552-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly24-Ala155
Molecular Weight	The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA (QC Test). The affinity constant of 2.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi Tag with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA (QC Test).
	Biotinylated Human IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.