Biotinylated Human IL-17A/CTLA-8 Protein (ILA-HM418B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test). The affinity constant of 2.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly24-Ala155

Catalog: ILA-HM418B

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Product Description Recombinant Biotinylated Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Ala155.
Product Category Recombinant Protein
Target IL-17A/CTLA-8
Synonyms CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A
Gene/Protein ID Q16552-1
Species Human
Exact Sequence Gly24-Ala155
Molecular Weight The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test). The affinity constant of 2.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi Tag with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test).
Biotinylated Human IL-17A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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