Name: Biotinylated Human LAG3/CD223 Protein (LAG-HM431B)
Brand: KactusBio
SKU: LAG-HM431B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve of biotinylated human LAG3 His tag protein showing mean absorbance versus log concentration

Biotinylated Human LAG3/CD223 Protein (LAG-HM431B)

Immobilized Biotinylated Human LAG3, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test).

Biotinylated Human LAG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human LAG3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human LAG3, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu23-Gly434

Catalog #LAG-HM431B

Size

100UG

500UG

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Biotinylated Human LAG3/CD223 Protein (LAG-HM431B) Summary

Product Description	Recombinant Biotinylated Human LAG3/CD223 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu23-Gly434.
Product Background	LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human LAG3/CD223 Protein (LAG-HM431B) Specifications/Details

Protein	LAG3/CD223
Synonyms	CD223; FDC; LAG3; LAG-3; sLAG-3
Accession	P18627-1
Species	Human
Biotinylated	yes
Amino Acid Range	Leu23-Gly434
Molecular Weight	The protein has a predicted MW of 47.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human LAG3, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PB, 150mM NaCl (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human LAG3, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-LAG3 Antibody, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test).
	Biotinylated Human LAG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human LAG3 is greater than 95% as determined by SEC-HPLC.