ELISA binding curve of Biotinylated Human Mature TGF beta 1, Avi Tag with Human TGF-beta RII, hFc Tag.
Biotinylated Human Mature TGF beta 1 Protein (TG1-HM10MB)
  • Express System: HEK293
  • Product Tag: C-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human TGF-beta RII, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala279-Ser390
Catalog #TG1-HM10MB
Regular price $660.00
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Biotinylated Human Mature TGF beta 1 Protein (TG1-HM10MB) Summary

Product Description

Recombinant Biotinylated Human Mature TGF beta 1 Protein is expressed from HEK293 with Avi tag at the C-Terminus.It contains Ala279-Ser390.

Product Background

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human Mature TGF beta 1 Protein (TG1-HM10MB) Specifications/Details

Protein
Mature TGF beta 1
Synonyms
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ala279-Ser390
Molecular Weight
The protein has a predicted MW of 13.2 kDa. Due to glycosylation, the protein migrates to 15-18 kDa under reduced (R) condition and 26-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity
Immobilized Human TGF-beta RII, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 50mM Glycine, 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human TGF-beta RII, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA. Immobilized Human TGF-beta RII, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA.
Biotinylated Human Mature TGF beta 1 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%. Biotinylated Human Mature TGF beta 1 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
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