Name: Biotinylated Human MMP-9 Protein (pro form) (MMP-HM409B)
Brand: KactusBio
SKU: MMP-HM409B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human MMP-9 Protein (pro form) (MMP-HM409B)

Immobilized Biotinylated Human MMP-9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA.

Biotinylated Human MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human MMP-9 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human MMP-9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 800 pmol/min/µg.
Amino Acid Range: Ala20-Asp707

Catalog #MMP-HM409B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human MMP-9 Protein (pro form) (MMP-HM409B) Summary

Product Description	Recombinant Biotinylated Human MMP-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. The protein needs to be activated by APMA for an activated form.It contains Ala20-Asp707.
Product Background	Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human MMP-9 Protein (pro form) (MMP-HM409B) Specifications/Details

Protein	MMP-9
Synonyms	MMP-9; GELB; CLG4B; MANDP2; Gelatinase B
Accession	P14780
Species	Human
Biotinylated	yes
Amino Acid Range	Ala20-Asp707
Molecular Weight	The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 95-105 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human MMP-9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 800 pmol/min/µg.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 10mM CaCl₂, 150mM NaCl, 0.05% Brij-35 (w/v) (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human MMP-9, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA.
	Biotinylated Human MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human MMP-9 is greater than 95% as determined by SEC-HPLC.