Name: Biotinylated Human Nectin-4 Protein IgV Domain (NEC-HM414B)
Brand: KactusBio
SKU: NEC-HM414B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human Nectin-4 Protein IgV Domain (NEC-HM414B)

Immobilized Biotinylated Human Nectin-4 IgV Domain, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test).

Biotinylated Human Nectin-4 IgV Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Nectin-4 IgV Domain is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Nectin-4 IgV Domain, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly32-Leu146

Catalog #NEC-HM414B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human Nectin-4 Protein IgV Domain (NEC-HM414B) Summary

Product Description	Recombinant Biotinylated Human Nectin-4 Protein IgV Domain is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Leu146.
Product Background	NECTIN-4 [a poliovirus receptor-related-4 (pvrl-4) encoded protein] is a Ca2+ independent immunoglobulin-like protein. Along with other Nectins (Nectin-1, -2 and -3), it is primarily involved in cell-cell adhesion. In contrast to other Nectins, Nectin-4 is specifically enriched in the embryonic and placental tissues but its expression significantly declines in adult life.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Biotinylated Human Nectin-4 Protein IgV Domain (NEC-HM414B) Specifications/Details

Protein	Nectin-4
Synonyms	EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Accession	Q96NY8-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly32-Leu146
Molecular Weight	The protein has a predicted MW of 15.8 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Nectin-4 IgV Domain, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human Nectin-4 IgV Domain, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test).
	Biotinylated Human Nectin-4 IgV Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Nectin-4 IgV Domain is greater than 95% as determined by SEC-HPLC.