Biotinylated Human Notch 1 Protein (NOT-HM401B)
Catalog: NOT-HM401B
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| Product Description | Recombinant Biotinylated Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala19-Gln526. |
Product Background |
Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. |
Product Category |
Recombinant Protein |
Target |
Notch 1 |
| Synonyms | Notch-1; TAN1; NOTCH1; 9930111A19Rik; lin-12; Mis6; N1 |
Gene/Protein ID |
P46531 |
Species |
Human |
Exact Sequence |
Ala19-Gln526 |
| Molecular Weight | The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Notch 1, His Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Human Notch 1 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Notch 1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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