Name: Biotinylated Human Notch 1 Protein (NOT-HM401B)
Brand: KactusBio
SKU: NOT-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human Notch 1 Protein (NOT-HM401B)

Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Notch 1, His Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human Notch 1, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).

Biotinylated Human Notch 1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Biotinylated Human Notch 1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Notch 1, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ala19-Gln526

Catalog #NOT-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human Notch 1 Protein (NOT-HM401B) Summary

Product Description	Recombinant Biotinylated Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala19-Gln526.
Product Background	Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human Notch 1 Protein (NOT-HM401B) Specifications/Details

Protein	Notch 1
Synonyms	Notch-1; TAN1; NOTCH1; 9930111A19Rik; lin-12; Mis6; N1
Accession	P46531
Species	Human
Biotinylated	yes
Amino Acid Range	Ala19-Gln526
Molecular Weight	The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Notch 1, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Notch 1, His Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human Notch 1, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).
	Biotinylated Human Notch 1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Biotinylated Human Notch 1 is greater than 95% as determined by SEC-HPLC.