Biotinylated Human Notch 1 Protein (NOT-HM401B)
Biotinylated Human Notch 1 Protein (NOT-HM401B)
Biotinylated Human Notch 1 Protein (NOT-HM401B)
Biotinylated Human Notch 1 Protein (NOT-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized BBiotinylated Human Notch 1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala19-Gln526
Catalog #NOT-HM401B
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Product Description
 Recombinant Biotinylated Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala19-Gln526.
Product Background
 Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Product Category
 Recombinant Protein
Target
 Notch 1
Synonyms
 Notch-1; TAN1; NOTCH1; 9930111A19Rik; lin-12; Mis6; N1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ala19-Gln526
Molecular Weight
 The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized BBiotinylated Human Notch 1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Notch 1, His Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
Immobilized BBiotinylated Human Notch 1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test).
Biotinylated Human Notch 1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human Notch 1 is greater than 95% as determined by SEC-HPLC.
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