Name: Biotinylated Human Notch 1 Protein (NOT-HM401B)
Brand: KactusBio
SKU: NOT-HM401B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human Notch 1 Protein (NOT-HM401B)

Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Notch 1, His Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human Notch 1, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).

Biotinylated Human Notch 1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Biotinylated Human Notch 1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Notch 1, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala19-Gln526

Catalog #NOT-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala19-Gln526.
Product Background	Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Product Category	Recombinant Protein / Antibody discovery

Protein	Notch 1
Synonyms	Notch-1; TAN1; NOTCH1; 9930111A19Rik; lin-12; Mis6; N1
Accession	P46531
Species	Human
Biotinylated	yes
Amino Acid Range	Ala19-Gln526
Molecular Weight	The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Notch 1, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Notch 1, His Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human Notch 1, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human DLL4, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).
	Biotinylated Human Notch 1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Biotinylated Human Notch 1 is greater than 95% as determined by SEC-HPLC.