Biotinylated Human Notch 3 Protein (NOT-HM403B)
Biotinylated Human Notch 3 Protein (NOT-HM403B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ala40-Glu467
Catalog #NOT-HM403B
Regular price $577.50
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Biotinylated Human Notch 3 Protein (NOT-HM403B) Summary

Product Description

Recombinant Biotinylated Human Notch 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala40-Glu467.

Product Background

Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human Notch 3 Protein (NOT-HM403B) Specifications/Details

Protein
Notch 3
Synonyms
CADASIL; Notch homolog 3; Notch-3; NOTCH3
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ala40-Glu467
Molecular Weight
The protein has a predicted MW of 47.7 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Product Tag
C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human Notch 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Notch 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Notch 3 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Notch 3 is greater than 95% as determined by SEC-HPLC.
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