Cynomolgus GDF15 Protein (GDF-CE115)

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Arg193-Val308

Catalog: GDF-CE115

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Product Description	Recombinant Cynomolgus GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Arg193-Val308.
Product Background	Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.
Product Category	Recombinant Protein

Target	GDF15
Gene/Protein ID	G7PWZ3
Species	Cynomolgus
Exact Sequence	Arg193-Val308
Molecular Weight	The protein has a predicted MW of 14.15 kDa. The protein migrates to 15-18 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA (QC Test).
	Immobilized Cynomolgus GDF15, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA.
	Cynomolgus GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.