Name: Human/Mouse/Rat Activin A Protein (ACV-HM001)
Brand: KactusBio
SKU: ACV-HM001-100UG
Price: 247.5 USD
Availability: InStock

Human/Mouse/Rat Activin A Protein (ACV-HM001)

Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human/Mouse/Rat Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).

Human ACVR1B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 18.43 nM as determined in SPR assay (Biacore T200).

Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test).

Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.

Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test).

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). The affinity constant of 55.35 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly311-Ser426

Catalog #ACV-HM001

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human/Mouse/Rat Activin A Protein (ACV-HM001) Summary

Product Description	Recombinant Human/Mouse/Rat Activin A Protein is expressed from HEK293 without tag.It contains Gly311-Ser426.
Product Background	Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human/Mouse/Rat Activin A Protein (ACV-HM001) Specifications/Details

Protein	Activin A
Synonyms	INHBA; Activin A; Inhibin beta A chain;
Accession	P08476
Species	Human/Mouse/Rat
Biotinylated	no
Amino Acid Range	Gly311-Ser426
Molecular Weight	The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). The affinity constant of 55.35 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human/Cynomolgus Activin RIIB, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human/Mouse/Rat Activin A, No Tag with an affinity constant of 55.35 pM as determined in SPR assay (Biacore T200).
	Human ACVR1B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Activin A, No Tag with an affinity constant of 18.43 nM as determined in SPR assay (Biacore T200).
	Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test).
	Immobilized Human/Mouse/Rat Activin A, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
	Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
	Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test).