Name: Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM103)
Brand: KactusBio
SKU: APE-HM103-100UG
Price: 346.5 USD
Availability: InStock

Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM103)

Immobilized Human Alkaline Phosphatase (Germ type), His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA.

Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Alkaline Phosphatase (Germ type) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Alkaline Phosphatase (Germ type), His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ile20-Asp503

Catalog #APE-HM103

Size

100UG

500UG

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Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM103) Summary

Product Description	Recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Ile20-Asp503.
Product Background	Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM103) Specifications/Details

Protein	Alkaline Phosphatase (Germ type)/ALPG
Synonyms	Alkaline Phosphatase; ALP-1; Alkaline phosphatase, placental-like; GCAP; PLAP-like; ALPG; ALPPL; ALPPL2
Accession	P10696
Species	Human
Biotinylated	no
Amino Acid Range	Ile20-Asp503
Molecular Weight	The protein has a predicted MW of 53.67 kDa. Due to glycosylation, the protein migrates to 58-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Alkaline Phosphatase (Germ type), His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human Alkaline Phosphatase (Germ type), His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA.
	Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Alkaline Phosphatase (Germ type) is greater than 95% as determined by SEC-HPLC.