Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM403)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human ANGPTL3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA (QC Test). The affinity constant of 17.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser17-Lys219
Catalog #ANG-HM403
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Product Description
 Recombinant Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser17-Lys219.
Product Background
 ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.
Product Category
 Recombinant Protein
Target
 ANGPTL3/Angiopoietin-like 3
Synonyms
 ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser17-Lys219
Molecular Weight
 The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ANGPTL3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA (QC Test). The affinity constant of 17.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB2 captured on Protein A chip, can bind Human ANGPTL3, His Tag with an affinity constant of 17.6nM as determined in a SPR assay (Biacore T200).
Immobilized Human ANGPTL3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA (QC Test).
Human ANGPTL3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

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