Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM403)
Catalog: ANG-HM403
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| Product Description | Recombinant Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser17-Lys219. |
Product Background |
ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. |
Product Category |
Recombinant Protein |
Target |
ANGPTL3/Angiopoietin-like 3 |
| Synonyms | ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3 |
Gene/Protein ID |
Q9Y5C1 |
Species |
Human |
Exact Sequence |
Ser17-Lys219 |
| Molecular Weight | The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human ANGPTL3, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. The affinity constant of 17.6nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human LILRB2 captured on Protein A chip, can bind Human ANGPTL3, His Tag with an affinity constant of 17.6nM as determined in a SPR assay (Biacore T200). |
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Immobilized Human ANGPTL3, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. |
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Human ANGPTL3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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