• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human ANGPTL3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. The affinity constant of 17.6nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ser17-Lys219
Catalog #ANG-HM403
Price
Regular price $264.00
Regular price Sale price $264.00
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Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM403) Summary

Product Description

 Recombinant Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser17-Lys219.

Product Background

 ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.

Non-biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM403) Specifications/Details

Protein
 ANGPTL3/Angiopoietin-like 3
Synonyms
 ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3;ANG5;Angiopoietin 5; ANGPT-5; ANGPT 5
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser17-Lys219
Molecular Weight
 The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human ANGPTL3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. The affinity constant of 17.6nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS, 0.05% CHAPS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human LILRB2 captured on Protein A chip, can bind Human ANGPTL3, His Tag with an affinity constant of 17.6nM as determined in a SPR assay (Biacore T200). Human LILRB2 captured on Protein A chip, can bind Human ANGPTL3, His Tag with an affinity constant of 17.6nM as determined in a SPR assay (Biacore T200).
Immobilized Human ANGPTL3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. Immobilized Human ANGPTL3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Human ANGPTL3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human ANGPTL3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...