Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM403)
Catalog: ANG-HM403
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Product Description | Recombinant Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ser17-Lys219. |
Product Category | Recombinant Protein |
Target | ANGPTL3/Angiopoietin-like 3 |
Synonyms | ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3 |
Gene/Protein ID | Q9Y5C1 |
Species | Human |
Exact Sequence | Ser17-Lys219 |
Molecular Weight | The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human ANGPTL3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. The affinity constant of 17.6nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | C-His-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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