Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM403)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human ANGPTL3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. The affinity constant of 17.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ser17-Lys219

Catalog: ANG-HM403

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Product Description Recombinant Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ser17-Lys219.
Product Category Recombinant Protein
Target ANGPTL3/Angiopoietin-like 3
Synonyms ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3
Gene/Protein ID Q9Y5C1
Species Human
Exact Sequence Ser17-Lys219
Molecular Weight The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human ANGPTL3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. The affinity constant of 17.6nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB2 captured on Protein A chip, can bind Human ANGPTL3, His Tag with an affinity constant of 17.6nM as determined in a SPR assay (Biacore T200).
Immobilized Human ANGPTL3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
Human ANGPTL3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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