Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM112) Summary
Product Description |
Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Thr141-Leu285 trimer design. |
Product Background |
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human BAFF/TNFSF13B/CD257 Trimer Protein (BAF-HM112) Specifications/Details
Protein |
BAFF/TNFSF13B/CD257 |
Synonyms |
BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr141-Leu285 |
Molecular Weight |
The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA (QC Test). The affinity constant of 6.59 nM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His-Flag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA (QC Test). |
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Immobilized Human BAFF Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA. |
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Immobilized Human BAFF Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BAFF Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. |
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Human BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human TACI, hFc Tag on ProA-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 1.53 nM as determined in BLI assay (Gator® Prime). |
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Loaded Biotinylated Human BAFFR, His-Avi Tag on Streptavidin-Biosensor can bind Human BAFF (Trimer), His-Flag Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator® Prime). |
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The purity of Human BAFF Trimer is greater than 95% as determined by SEC-HPLC. |
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