Name: Human BDCA-2 Protein (BCA-HM102)
Brand: KactusBio
SKU: BCA-HM102-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Phe46-Ile213

Catalog #BCA-HM102

Size

100UG

500UG

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Human BDCA-2 Protein (BCA-HM102) Summary

Product Description	Recombinant Human BDCA-2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Phe46-Ile213.
Product Background	BDCA-2 is a novel type II C-type lectin specifically expressed by plasmacytoid dendritic cells (PDCs) that can internalize antigen for presentation to T cells. Furthermore, signaling via BDCA-2 may play a role in switching from interferon (IFN)-α/β-controlled to interleukin (IL)-12-controlled immune response pathways, as triggering of BDCA-2 potently inhibits secretion of IFN-α/β by PDCs and thereby promotes IL-12 p70 production in PDCs and other cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human BDCA-2 Protein (BCA-HM102) Specifications/Details

Protein	BDCA-2
Synonyms	BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303;
Accession	Q8WTT0-1
Species	Human
Biotinylated	no
Amino Acid Range	Phe46-Ile213
Molecular Weight	The protein has a predicted MW of 20.93 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 20mM Tris, 500mM NaCl, 200mM L-arginine (pH 8.5).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-BDCA-2 Antibody captured on CM5 Chip via Protein A can bind Human BDCA-2, His Tag with an affinity constant of 2.97 nM as determined in SPR assay (Biacore T200) (Routine Test).
	Immobilized Human BDCA-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test).
	Human BDCA-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.