Name: Human CCL5 Protein (CCL-HE105)
Brand: KactusBio
SKU: CCL-HE105-100UG
Price: 330.0 USD
Availability: InStock

Express System: E.coli
Product Tag: N-His,SUMO
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.77 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser24-Ser91

Catalog #CCL-HE105

Size

100UG

500UG

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Human CCL5 Protein (CCL-HE105) Summary

Product Description	Recombinant Human CCL5 Protein is expressed from E.coli with His-SUMO tag at the N-Terminus.It contains Ser24-Ser91.
Product Background	The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human CCL5 Protein (CCL-HE105) Specifications/Details

Protein	CCL5
Synonyms	MuRantes; SIS-delta; Scya5; Ccl5; D17S136E; eoCP; RANTES; SCYA5; SISd; TCP228; CCL5
Accession	P13501
Species	Human
Biotinylated	no
Amino Acid Range	Ser24-Ser91
Molecular Weight	The protein has a predicted MW of 21 kDa. Due to the protein can cleaved into two chains, so it migrates to 15-18 kDa and 22-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.77 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His,SUMO
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris,0.3M NaCl (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human GPR75 Nanodisc captured on CM5 Chip via Streptavidin can bind Human CCL5, His Tag with an affinity constant of 0.77 nM as determined in SPR assay (Biacore T200).
	Immobilized Human CCL5, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test).
	Immobilized Human CCL5, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1ug/ml determined by ELISA.
	Human CCL5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.