- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human Complement C5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test).
- Amino Acid Range: Gln19-Cys1676
Human Complement C5 Protein (CC5-HM1C5) Summary
Product Description |
Recombinant Human Complement C5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Cys1676. |
Product Background |
Complement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates.C5 plays a major role in complement activation; C5 convertase cleaves C5 into the pro-inflammatory C5a, and C5b, the nidus for the formation of the lytic membrane attack complex. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Complement C5 Protein (CC5-HM1C5) Specifications/Details
Protein |
Complement C5 |
Synonyms |
Complement C5; C5; CPAMD4; ECLZB; C5a; C5b; C5D |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln19-Cys1676 |
Molecular Weight |
Human Complement C5 Protein is cleaved into α chain and β chain by the enzyme lysis. Each chain has a predicted MW of 112.5 kDa (α chain) and 73.29 (β chain). Due to glycosylation, the protein migrates to 110-114 kDa (α chain) and 69-73 (β chain) based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Complement C5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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