Human DLL3 Domain (311-479) Protein (DLL-HM4D1)
Human DLL3 Domain (311-479) Protein (DLL-HM4D1)
Human DLL3 Domain (311-479) Protein (DLL-HM4D1)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human DLL3 Domain (311-479), His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val311-Ala479
Catalog #DLL-HM4D1
Regular price $300.00
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Product Description
Recombinant Human DLL3 Domain (311-479) Protein is expressed from HEK293 with with His tag and Avi tag at the C-Terminus.It contains Val311-Ala479.
Product Background
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
Product Category
Recombinant Protein
Target
DLL3 Domain (311-479)
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Val311-Ala479
Molecular Weight
The protein has a predicted MW of 21.20 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human DLL3 Domain (311-479), His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human DLL3 Domain (311-479), His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
Human DLL3 Domain (311-479) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human DLL3 Domain (311-479) is greater than 95% as determined by SEC-HPLC.

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