Name: Non-biotinylated Human DLL3 Domain (311-479) Protein (DLL-HM4D1)
Brand: KactusBio
SKU: DLL-HM4D1-100UG
Price: 330.0 USD
Availability: InStock

ELISA binding curve showing the interaction of anti-DLL3 antibody with human DLL3 Domain 3–6 containing a His tag.

Non-biotinylated Human DLL3 Domain (311-479) Protein (DLL-HM4D1)

Immobilized Human DLL3 Domain (311-479), His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA.

Human DLL3 Domain (311-479) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-DLL3 Antibody, hFc Tag on ProA-Biosensor can bind Human DLL3 (311-479), His-Avi Tag with an affinity constant of 1.14 nM as determined in BLI assay (Gator® Prime).

The purity of Human DLL3 Domain (311-479) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human DLL3 Domain (311-479), His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Val311-Ala479

Catalog #DLL-HM4D1

Size

100UG

500UG

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Product Description	Recombinant Human DLL3 Domain (311-479) Protein is expressed from HEK293 with with His tag and Avi tag at the C-Terminus.It contains Val311-Ala479.
Product Background	Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	DLL3
Synonyms	Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3
Accession	Q9NYJ7-1
Species	Human
Biotinylated	no
Amino Acid Range	Val311-Ala479
Molecular Weight	The protein has a predicted MW of 21.20 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human DLL3 Domain (311-479), His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human DLL3 Domain (311-479), His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA.
	Human DLL3 Domain (311-479) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-DLL3 Antibody, hFc Tag on ProA-Biosensor can bind Human DLL3 (311-479), His-Avi Tag with an affinity constant of 1.14 nM as determined in BLI assay (Gator® Prime).
	The purity of Human DLL3 Domain (311-479) is greater than 95% as determined by SEC-HPLC.

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