Human DLL3 Domain (311-479) Protein (DLL-HM4D1)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human DLL3 Domain (311-479) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val311-Ala479

Catalog: DLL-HM4D1

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Product Description Recombinant Human DLL3 Domain (311-479) Protein is expressed from HEK293 with with His tag and Avi tag at the C-Terminus.It contains Val311-Ala479.

Product Background

Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

Product Category

Recombinant Protein

Target

DLL3 Domain (311-479)

Gene/Protein ID

Q9NYJ7-1

Species

Human

Exact Sequence

Val311-Ala479
Molecular Weight The protein has a predicted MW of 21.20 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human DLL3 Domain (311-479) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human DLL3 Domain (311-479) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
The purity of Human DLL3 Domain (311-479) is greater than 95% as determined by SEC-HPLC.
Human DLL3 Domain (311-479) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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