Human EGFRVIII Protein (EG8-HM154)
Catalog: EG8-HM154
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| Product Description | Recombinant Human EGFRVIII Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Leu25-Ser378. |
| Product Category | Recombinant Protein |
| Target | EGFRVIII |
| Synonyms | ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
| Gene/Protein ID | NP_001333870.1 |
| Species | Human |
| Exact Sequence | Leu25-Ser378 |
| Molecular Weight | The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human EGFRVIII, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human EGFRVIII, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. |
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The purity of Human EGFRVIII is greater than 95% as determined by SEC-HPLC. |
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Human EGFRVIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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