Name: Non-biotinylated Human EGFRVIII Protein (EG8-HM154)
Brand: KactusBio
SKU: EG8-HM154-100UG
Price: 330.0 USD
Availability: InStock

ELISA binding curve showing the dose-dependent interaction between Human EGFRVIII, His Tag, and Anti-EGFRVIII Antibody, hFc Tag. The plot presents mean absorbance (OD450) versus the log concentration of the antibody (µg/ml), demonstrating a sigmoidal response.

Non-biotinylated Human EGFRVIII Protein (EG8-HM154)

Immobilized Human EGFRVIII, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA.

Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human EGFRVIII is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EGFRVIII, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Leu25-Ser378

Catalog #EG8-HM154

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Non-biotinylated Human EGFRVIII Protein (EG8-HM154) Summary

Product Description	Recombinant Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser378.
Product Background	The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Non-biotinylated Human EGFRVIII Protein (EG8-HM154) Specifications/Details

Protein	EGFRVIII
Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession	NP_001333870.1
Species	Human
Biotinylated	no
Amino Acid Range	Leu25-Ser378
Molecular Weight	The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human EGFRVIII, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human EGFRVIII, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA.
	Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human EGFRVIII is greater than 95% as determined by SEC-HPLC.