Name: Human FGF basic Protein (FGF-HE001)
Brand: KactusBio
SKU: FGF-HE001-100UG
Price: 74.8 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human FGF basic at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 11.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Pro143-Ser288

Catalog #FGF-HE001

Size

100UG

500UG

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Human FGF basic Protein (FGF-HE001) Summary

Product Description	Recombinant Human FGF basic Protein is expressed from E.coli without tag; It contains Pro143-Ser288.
Product Background	FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human FGF basic Protein (FGF-HE001) Specifications/Details

Protein	FGF basic
Synonyms	bFGF; FGF basic; FGF2; FGF-2; FGF2AS; GFG1; HBGF-2; NUDT6; Prostatropin; BFGF; FGFB; FGFβ; HBGF2
Accession	P09038-4
Species	Human
Biotinylated	no
Amino Acid Range	Pro143-Ser288
Molecular Weight	The protein has a predicted MW of 16.5 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human FGF basic at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 11.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 0.02 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human FGF basic at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 11.1ng/ml determined by ELISA.
	Immobilized Human FGF basic, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human FGFR2 beta (IIIc), His Tag with the EC50 of 0.49 ug/ml determined by ELISA.
	Human FGF basic on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50-for this effect is 0.1 - 0.6 ng/ml (QC Test).