Name: Human FLT3 Ligand Protein (FLT-HM13L)
Brand: KactusBio
SKU: FLT-HM13L-100UG
Price: 363.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test).
Amino Acid Range: Thr27-Pro185

Catalog #FLT-HM13L

Size

100UG

500UG

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Human FLT3 Ligand Protein (FLT-HM13L) Summary

Product Description	Recombinant Human FLT3 Ligand Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr27-Pro185.
Product Background	Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human FLT3 Ligand Protein (FLT-HM13L) Specifications/Details

Protein	FLT3 Ligand
Synonyms	FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine; Flt-3 Ligand; Flt-3L
Accession	P49771-1
Species	Human
Biotinylated	no
Amino Acid Range	Thr27-Pro185
Molecular Weight	The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human FLT3 Ligand, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test).
	Human FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 2.0 ng/ml.
	The purity of Human FLT3 Ligand is greater than 95% as determined by SEC-HPLC.