Product Description |
Recombinant Human FOLR4/Juno Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Ser228. |
Product Background |
Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor. |
Product Category |
Recombinant Protein |
Target |
FOLR4/Juno |
Synonyms |
Folate receptor 4; FR-delta; FR delta; Izumo1r; Folbp3; Juno; FOLR4, LOC390243 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly20-Ser228 |
Molecular Weight |
The protein has a predicted MW of 25 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human FOLR4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human FOLR4 is greater than 95% as determined by SEC-HPLC. |
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