Name: Non-biotinylated Human GPA33/A33 Protein (GPA-HM433)
Brand: KactusBio
SKU: GPA-HM433-100UG
Price: 247.5 USD
Availability: InStock

Non-biotinylated Human GPA33/A33 Protein (GPA-HM433)

Anti-GPA33 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human GPA33, His Tag with an affinity constant of 0.69 nM as determined in SPR assay (Biacore T200).

Immobilized Human GPA33, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).

Human GPA33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human GPA33 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GPA33, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.69 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ile22-Val235

Catalog #GPA-HM433

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Non-biotinylated Human GPA33/A33 Protein (GPA-HM433) Summary

Product Description	Recombinant Human GPA33/A33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile22-Val235.
Product Background	Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Non-biotinylated Human GPA33/A33 Protein (GPA-HM433) Specifications/Details

Protein	GPA33/A33
Synonyms	Glycoprotein A33; GPA33; A33; MGC129986; MGC129987
Accession	Q99795-1
Species	Human
Biotinylated	no
Amino Acid Range	Ile22-Val235
Molecular Weight	The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GPA33, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.69 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Anti-GPA33 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human GPA33, His Tag with an affinity constant of 0.69 nM as determined in SPR assay (Biacore T200).
	Immobilized Human GPA33, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
	Human GPA33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human GPA33 is greater than 95% as determined by SEC-HPLC.