Human GPA33/A33 Protein (GPA-HM433)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GPA33, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. The affinity constant of 0.69 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ile22-Val235

Catalog: GPA-HM433

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Product Description	Recombinant Human GPA33/A33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile22-Val235.
Product Background	Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
Product Category	Recombinant Protein

Target	GPA33/A33
Synonyms	Glycoprotein A33; GPA33; A33; MGC129986; MGC129987
Gene/Protein ID	Q99795-1
Species	Human
Exact Sequence	Ile22-Val235
Molecular Weight	The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human GPA33, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. The affinity constant of 0.69 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Anti-GPA33 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human GPA33, His Tag with an affinity constant of 0.69 nM as determined in SPR assay (Biacore T200).
	Immobilized Human GPA33, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
	The purity of Human GPA33 is greater than 95% as determined by SEC-HPLC.
	Human GPA33 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.