Product Description |
Recombinant Human GPA33/A33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile22-Val235. |
Product Background |
Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling. |
Product Category |
Recombinant Protein |
Target |
GPA33/A33 |
Synonyms |
Glycoprotein A33; GPA33; A33; MGC129986; MGC129987 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile22-Val235 |
Molecular Weight |
The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GPA33, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.69 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Anti-GPA33 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human GPA33, His Tag with an affinity constant of 0.69 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human GPA33, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). |
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Human GPA33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human GPA33 is greater than 95% as determined by SEC-HPLC. |
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