Name: Human HGF Protein (HGF-HM201)
Brand: KactusBio
SKU: HGF-HM201-100UG
Price: 379.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HGF R, His Tag at 2ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gln32-Ser728

Catalog #HGF-HM201

Size

100UG

500UG

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Human HGF Protein (HGF-HM201) Summary

Product Description	Recombinant Human HGF Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Gln32-Ser728.
Product Background	Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types; plays a key role in β-cell homeostasis; and is capable of modulating the inflammatory response.HGF plays a central role in these metabolic disorders,HGF levels could be employed as a biomarker for disease status/progression, and HGF/c-Met signaling pathway modulators could effectively regulate IR and treat diabetes.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human HGF Protein (HGF-HM201) Specifications/Details

Protein	HGF
Synonyms	Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB
Accession	P14210-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln32-Ser728
Molecular Weight	The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
Activity	Immobilized Human HGF R, His Tag at 2ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 100 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human HGF R, His Tag at 2ug/ml (100 ul/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 10.0 ng/ml determined by ELISA (QC Test).
	Human HGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human HGF, hFc Tag on ProA-Biosensor, can bind Human HGF R, His-Avi Tag with an affinity constant of 9.93 nM as determined in BLI assay (Gator® Prime).
	The purity of Human HGF is greater than 95% as determined by SEC-HPLC.