Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein (MHC-HM406T) Summary
Product Description |
Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide. |
Product Background |
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Specifications
Protein |
HLA-E*01:03&B2M&Peptide (VMAPRTLVL) |
Synonyms |
HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1 |
MHC Form |
Tetramer |
Allele |
HLA-E*01:03 |
Accession |
P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide |
Molecular Weight |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test). The affinity constant of 17.61 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human NKG2A&CD94, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 17.61 nM as determined in SPR assay (Biacore T200). |
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Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test). |
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Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%. |
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The purity of Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer is greater than 95% as determined by SEC-HPLC. |
Additional Information
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