Name: Human IDH1 Protein (IDH-HE101)
Brand: KactusBio
SKU: IDH-HE101-100UG
Price: 297.0 USD
Availability: InStock

Express System: E.coli
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10000 pmol/min/ug.
Amino Acid Range: Ser2-Leu414

Catalog #IDH-HE101

Size

100UG

500UG

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Human IDH1 Protein (IDH-HE101) Summary

Product Description	Recombinant Human IDH1 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Ser2-Leu414.
Product Background	Isocitrate dehydrogenase (IDH) is a metabolic enzyme that converts isocitrate to α-ketoglutarate (α-KG). Genetic gain-of-function mutations in IDH1 and IDH2 confer a neomorphic activity that allow reduction of α -KG to (R)-2- hydroxyglutarate, the accumulation of which results in the development of cancers like low grade gliomas and leukemia.Isocitrate dehydrogenase 1 (IDH1) is the most frequently mutated metabolic gene in cancer.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human IDH1 Protein (IDH-HE101) Specifications/Details

Protein	IDH1
Synonyms	IDH; IDH1; IDPc; PICD; IDP; DCD; IDH; Isocitrate dehydrogenase; IDCD;
Accession	O75874
Species	Human
Biotinylated	no
Amino Acid Range	Ser2-Leu414
Molecular Weight	The protein has a predicted MW of 47.35 kDa same as Bis-Tris PAGE result.
Activity	Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10000 pmol/min/ug.
Product Tag	C-His
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 50mM Tris, 200mM NaCl, 1mM DTT, 10% Glycerol (pH 7.5).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IDH1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IDH1 is greater than 95% as determined by SEC-HPLC.