Name: Human IFN alpha 1 Protein (IFN-HM2A1)
Brand: KactusBio
SKU: IFN-HM2A1-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Cys24-Glu189

Catalog #IFN-HM2A1

Size

100UG

500UG

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Human IFN alpha 1 Protein (IFN-HM2A1) Summary

Product Description	Recombinant Human IFN alpha 1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys24-Glu189.
Product Background	IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human IFN alpha 1 Protein (IFN-HM2A1) Specifications/Details

Protein	IFN alpha 1
Synonyms	Interferon alpha-1/13;IFN-alpha-1/13;Interferon alpha-D;LeIF D; IFNalpha 1; IFN alpha1;IFNA1;IFN-alpha-1;Interferon alpha 1
Accession	P01562
Species	Human
Biotinylated	no
Amino Acid Range	Cys24-Glu189
Molecular Weight	The protein has a predicted MW of 46.14 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200) (QC Test).
	Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200).
	Human IFN alpha 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human IFN alpha/beta R2, His Tag on Anti-His-Biosensor, can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.10 nM as determined in BLI assay (Gator).
	The purity of Human IFN alpha 1 is greater than 95% as determined by SEC-HPLC.