Name: Human IL-1 alpha/IL-1A Protein (IL1-HE00A)
Brand: KactusBio
SKU: IL1-HE00A-100UG
Price: 346.5 USD
Availability: InStock

HPLC chromatogram showing a sharp peak at 9.702 minutes, used for product validation of a recombinant protein sample, indicating high purity.

Human IL-1 alpha/IL-1A Protein (IL1-HE00A)

Human IL-1R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).

Human IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser113-Ala271

Catalog #IL1-HE00A

Size

100UG

500UG

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Human IL-1 alpha/IL-1A Protein (IL1-HE00A) Summary

Product Description	Recombinant Human IL-1 alpha/IL-1A Protein is expressed from E.coli without tag.It contains Ser113-Ala271.
Product Background	The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human IL-1 alpha/IL-1A Protein (IL1-HE00A) Specifications/Details

Protein	IL-1 alpha/IL1A
Synonyms	Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1
Accession	NP_000566.3
Species	Human
Biotinylated	no
Amino Acid Range	Ser113-Ala271
Molecular Weight	The protein has a predicted MW of 18 kDa same as Bis-Tris PAGE result.
Activity	The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.05 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-1R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).
	Human IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.