Human IL-1 Beta/IL-1F2 Protein (IL1-HE40B)

  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
  • Activity: Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1 RII, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala117-Ser269

Catalog: IL1-HE40B

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Product Description Recombinant Human IL-1 Beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-terminal.; It contains Ala117-Ser269.
Product Category Recombinant Protein
Target IL-1 Beta/IL-1F2
Synonyms Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B
Gene/Protein ID P01584
Species Human
Exact Sequence Ala117-Ser269
Molecular Weight The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1 RII, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1 RII, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA.
The purity of Human IL-1 Beta/IL-1F2 is greater than 95% as determined by SEC-HPLC.
Human IL-1 Beta/IL-1F2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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