Name: Non-biotinylated Human IL-1 Beta/IL-1F2 Protein (IL1-HE40B)
Brand: KactusBio
SKU: IL1-HE40B-100UG
Price: 297.0 USD
Availability: InStock

Non-biotinylated Human IL-1 Beta/IL-1F2 Protein (IL1-HE40B)

Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).

Human IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human IL-1 Beta, His-Avi Tag on Anti-His-Biosensor, can bind Human IL-1R1, No Tag with an affinity constant of 9.42 nM as determined in BLI assay (Gator).

The purity of Human IL-1 Beta is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala117-Ser269

Catalog #IL1-HE40B

Size

100UG

500UG

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Non-biotinylated Human IL-1 Beta/IL-1F2 Protein (IL1-HE40B) Summary

Product Description	Recombinant Human IL-1 Beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-Terminus.It contains Ala117-Ser269.
Product Background	Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Non-biotinylated Human IL-1 Beta/IL-1F2 Protein (IL1-HE40B) Specifications/Details

Protein	IL-1 Beta/IL-1F2
Synonyms	Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B ;Interleukin-1 β; IL-1 β; IL-1β; IL-1 β
Accession	P01584
Species	Human
Biotinylated	no
Amino Acid Range	Ala117-Ser269
Molecular Weight	The protein has a predicted MW of 20.1 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
	Human IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human IL-1 Beta, His-Avi Tag on Anti-His-Biosensor, can bind Human IL-1R1, No Tag with an affinity constant of 9.42 nM as determined in BLI assay (Gator).
	The purity of Human IL-1 Beta is greater than 95% as determined by SEC-HPLC.