Name: Human IL-10 Protein (IL1-HM010)
Brand: KactusBio
SKU: IL1-HM010-100UG
Price: 412.5 USD
Availability: InStock

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.255 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser19-Asn178

Catalog #IL1-HM010

Size

100UG

500UG

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Human IL-10 Protein (IL1-HM010) Summary

Product Description	Recombinant Human IL-10 Protein is expressed from HEK293 without tag.It contains Ser19-Asn178.
Product Background	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human IL-10 Protein (IL1-HM010) Specifications/Details

Protein	IL-10
Synonyms	Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451
Accession	P22301
Species	Human
Biotinylated	no
Amino Acid Range	Ser19-Asn178
Molecular Weight	The protein has a predicted MW of 18.8 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.255 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-10, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9ng/ml determined by ELISA (QC Test).
	Human IL-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to inhibit IL-6 secretion by RAW264.7 cells. The ED50 for this effect is 0.1 -1.0 ng/mL (QC Test).
	The purity of Human IL-10 is greater than 95% as determined by SEC-HPLC.