Human IL-13Ra1 Protein (ILR-HM2R1)
Human IL-13Ra1 Protein (ILR-HM2R1)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 9.47 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly22-Thr343
Catalog #ILR-HM2R1
Regular price $181.50
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Human IL-13Ra1 Protein (ILR-HM2R1) Summary

Product Description

Recombinant Human IL-13Ra1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly22-Thr343.

Product Background

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Human IL-13Ra1 Protein (ILR-HM2R1) Specifications/Details

Protein
IL-13Ra1
Synonyms
IL-13 R alpha 1; IL13R; IL13RA; CT19; IL13RA1; NR4; CD213A1; AI882074
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly22-Thr343
Molecular Weight
The protein has a predicted MW of 63.4 kDa. Due to glycosylation, the protein migrates to 75-110 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 9.47 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13, His Tag with an affinity constant of 9.47 nM as determined in SPR assay (Biacore T200). Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13, His Tag with an affinity constant of 9.47 nM as determined in SPR assay (Biacore T200).
Human IL-13Ra1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human IL-13Ra1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Loaded Human IL-13, His Tag on Anti-His-Biosensor can bind Human IL-13Ra1, hFc Tag with an affinity constant of 40.70 nM as determined in BLI assay (Gator® Prime). Loaded Human IL-13, His Tag on Anti-His-Biosensor can bind Human IL-13Ra1, hFc Tag with an affinity constant of 40.70 nM as determined in BLI assay (Gator® Prime).
The purity of-Human IL-13Ra1 is greater than 95% as determined by SEC-HPLC. The purity of-Human IL-13Ra1 is greater than 95% as determined by SEC-HPLC.
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