Human IL-5 R alpha/CD125 Protein (ILR-HM25R)
Catalog: ILR-HM25R
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| Product Description | Recombinant Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp21-Glu335. |
| Product Category | Recombinant Protein |
| Target | IL-5 R alpha/CD125 |
| Synonyms | CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560 |
| Gene/Protein ID | Q01344-1 |
| Species | Human |
| Exact Sequence | Asp21-Glu335 |
| Molecular Weight | The protein has a predicted MW of 62.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human IL-5, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. |
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The purity of Human IL-5R alpha is greater than 95% as determined by SEC-HPLC. |
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Human IL-5R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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