Human IL-5 R alpha/CD125 Protein (ILR-HM25R)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-5, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp21-Glu335

Catalog: ILR-HM25R

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Product Description Recombinant Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp21-Glu335.

Product Background

Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.

Product Category

Recombinant Protein

Target

IL-5 R alpha/CD125
Synonyms CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560
Accession

Species

Human

Amino Acid Range

Asp21-Glu335
Molecular Weight The protein has a predicted MW of 62.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-5, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-5, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.
The purity of Human IL-5R alpha is greater than 95% as determined by SEC-HPLC.
Human IL-5R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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