Name: Human IL-5 R alpha/CD125 Protein (ILR-HM25R)
Brand: KactusBio
SKU: ILR-HM25R-100UG
Price: 297.0 USD
Availability: InStock

Human IL-5 R alpha/CD125 Protein (ILR-HM25R)

ELISA binding curve showing a dose-dependent increase in absorbance (OD450) as Human IL-5 R alpha concentration increases, indicating binding to IL-5.

Immobilized Human IL-5, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.

Human IL-5R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-5R alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-5, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.
Amino Acid Range: Asp21-Glu335
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Catalog #ILR-HM25R

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human IL-5 R alpha/CD125 Protein (ILR-HM25R) Summary

Product Description	Recombinant Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Asp21-Glu335.
Product Background	Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human IL-5 R alpha/CD125 Protein (ILR-HM25R) Specifications/Details

Protein	IL-5 R alpha/CD125
Synonyms	CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560
Accession	Q01344-1
Species	Human
Biotinylated	no
Amino Acid Range	Asp21-Glu335
Molecular Weight	The protein has a predicted MW of 62.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-5, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human IL-5, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.
	Human IL-5R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-5R alpha is greater than 95% as determined by SEC-HPLC.