Non-biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein (ITG-HM4V6) Summary
Product Description |
Recombinant Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992(ITGAV) acidic tail and Gly22-Asn707(ITGB6) basic tail. |
Product Background |
ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein (ITG-HM4V6) Specifications/Details
Protein |
Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer |
Synonyms |
αVβ6; Integrin alpha V beta 6; ITGAV&ITGB6;Integrin alpha V β 6 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe31-Val992(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail |
Molecular Weight |
The protein has a predicted MW of 114 kDa (ITGAV)&79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-150 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ITGAV&ITGB6, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 63.3 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Human ITGAV&ITGB6, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 63.3 ng/ml determined by ELISA (QC Test). |
|
|
Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB6 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. |
|
|
Immobilized Human ITGAV&ITGB6, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGAV&ITGB6 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA. |
|
|
Human ITGAV&ITGB6 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
|
|
Loaded Anti-ITGAV&ITGB6 Antibody, hFc Tag on ProA-Biosensor can bind Human ITGAV&ITGB6, His Tag with an affinity constant of 18.20 nM as determined in BLI assay (Gator® Prime). |
|
|
Loaded Anti-ITGB6 Antibody, hFc Tag on ProA-Biosensor can bind Human ITGAV&ITGB6, His Tag with an affinity constant of 92 nM as determined in BLI assay (Gator® Prime). |
|
|
The purity of Human ITGAV&ITGB6 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed
