Human NTS1 Protein (NTS-HM201) Summary
Product Description |
Recombinant Human NTS1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ser24-Leu163. |
Product Background |
Neurotensin (NT) is an endogenous 13 amino acid neuropeptide with profound opioid-independent analgesic effects. This role of NT is thought to be mediated by both neurotensin receptor subtype 1 (NTS1) and neurotensin receptor subtype 2 (NTS2). NT and its receptors are widely distributed in the pain circuits in central nervous system. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human NTS1 Protein (NTS-HM201) Specifications/Details
Protein |
NTS1 |
Synonyms |
NT; NTR; NTR1; NTRH; NTRR; NTS1; NTSR1; NMN-125; NN; NT/N; NTS; Neurotensin/neuromedin N; Neurotensin |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser24-Leu163 |
Molecular Weight |
The protein has a predicted MW of 42.9 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NTS1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human NTS1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA. |
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Human NTS1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human NTS1 is greater than 95% as determined by SEC-HPLC. |
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