Human NTS1 Protein (NTS-HM201)
Human NTS1 Protein (NTS-HM201)
Human NTS1 Protein (NTS-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NTS1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser24-Leu163
Catalog #NTS-HM201
Regular price $297.00
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Product Description

Recombinant Human NTS1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser24-Leu163.

Product Background

Neurotensin (NT) is an endogenous 13 amino acid neuropeptide with profound opioid-independent analgesic effects. This role of NT is thought to be mediated by both neurotensin receptor subtype 1 (NTS1) and neurotensin receptor subtype 2 (NTS2). NT and its receptors are widely distributed in the pain circuits in central nervous system.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

NTS1

Synonyms

NT; NTR; NTR1; NTRH; NTRR; NTS1; NTSR1; NMN-125; NN; NT/N; NTS; Neurotensin/neuromedin N; Neurotensin

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ser24-Leu163

Molecular Weight

The protein has a predicted MW of 42.9 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human NTS1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human NTS1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA. Immobilized Human NTS1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA.
Human NTS1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human NTS1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human NTS1 is greater than 95% as determined by SEC-HPLC. The purity of Human NTS1 is greater than 95% as determined by SEC-HPLC.

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