Product Description |
Recombinant Human NTS1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser24-Leu163. |
Product Background |
Crystal structures of neurotensin receptor subtype 1 (NTS1) allowed us to visualize the binding mode of the endogenous peptide hormone neurotensin and its pharmacologically active C-terminal fragment NT(8-13) within the orthosteric binding pocket of NTS1. |
Product Category |
Recombinant Protein |
Target |
NTS1 |
Synonyms |
NT; NTR; NTR1; NTRH; NTRR; NTS1; NTSR1; NMN-125; NN; NT/N |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser24-Leu163 |
Molecular Weight |
The protein has a predicted MW of 42.9 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NTS1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
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Immobilized Human NTS1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.21ug/ml determined by ELISA. |
|
|
Human NTS1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human NTS1 is greater than 95% as determined by SEC-HPLC. |
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