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Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer Protein (MHC-HM415T) Summary
Product Description |
Recombinant Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and HMTEVVRHC peptide. |
Product Background |
p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Specifications
Protein |
HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) |
Synonyms |
MHC; HLA-A; P53; TP53; Antigen NY-CO-13; BCC7; FLJ92943; LFS1; TRP53 |
MHC Form |
Tetramer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRHC |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and HMTEVVRHC peptide |
Molecular Weight |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA. |
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Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%. |
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The purity of Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Tetramer is greater than 95% as determined by SEC-HPLC. |
Additional Information
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