Product Description |
Recombinant Human PAH (D415N) Protein is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the N-terminus.It contains Met1-Lys452 (D415N). |
Product Background |
Phenylalanine hydroxylase (PAH) is a member of aromatic amino acid hydroxylase (AAAHs) family, and catalyze phenylalanine (Phe) into tyrosine (Tyr). PAH is also an allosteric enzyme that maintains phenylalanine (Phe) below neurotoxic levels; its failure results in phenylketonuria, an inborn error of amino acid metabolism. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
PAH (D415N) |
Synonyms |
PH; PKU; PKU1; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Lys452 (D415N) |
Molecular Weight |
The protein has a predicted MW of 52.82 kDa same as Bis-Tris PAGE result. |
Product Tag |
N-His |
Expression System |
Baculovirus-Insect Cells(Sf9) |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM Tris, 0.5M NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human PAH (D415N) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human PAH (D415N) is greater than 95% as determined by SEC-HPLC. |
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