Name: Human PLD4 Protein (PLD-HM104)
Brand: KactusBio
SKU: PLD-HM104-100UG
Price: 297.0 USD
Availability: InStock

Catalog #PLD-HM104

Size

100UG

500UG

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Human PLD4 Protein (PLD-HM104) Summary

Product Description	Recombinant Human PLD4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp52-Gly506.
Product Background	Phospholipase D4 (PLD4) is a newly identified protein expressed in microglia. the expression of PLD4 was located in macrophages in the colon cancer mesenchymal and lymph nodes as shown by immunohistochemical analysis. furthermore, its expression was associated with clinical staging of colon cancer. Then, THP-1 as a cell model induced into TAMs. PLD4 could be involved in the activation process of M1 phenotype macrophages.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human PLD4 Protein (PLD-HM104) Specifications/Details

Protein	PLD4
Synonyms	5'-3' exonuclease PLD4; Choline phosphatase 4; Phospholipase D4; PLD 4
Accession	Q96BZ4
Species	Human
Biotinylated	no
Amino Acid Range	Trp52-Gly506
Molecular Weight	The protein has a predicted MW of 51.1 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM MES, 100mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 50mM MES, 100mM NaCl (pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human PLD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human PLD4 is greater than 95% as determined by SEC-HPLC.