Product Description |
Recombinant Monkeypox virus E8L Protein is expressed from E.coli with His tag at the N-Terminus.It contains Met1-Thr275. |
Product Background |
E8L is an important protein that mediates the invasion of monkeypox virus into host cells. In the process of invading and completing replication, E8L acts as a surface-binding protein of mature virion and can bind to chondroitin sulfate on the cell surface, so that the virus can attach to target cells. |
Product Category |
Recombinant Protein |
Target |
E8L |
Synonyms |
Cell surface-binding protein; Carbonic anhydrase homolog |
Accession |
|
Species |
Monkeypox virus |
Biotinylated |
no |
Amino Acid Range |
Met1-Thr275 |
Molecular Weight |
The protein has a predicted MW of 32.92 kDa. The protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Monkeypox virus E8L on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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