Name: Mouse FLT3 Ligand Protein (FLT-MM23L)
Brand: KactusBio
SKU: FLT-MM23L-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test). The affinity constant of 13.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly27-Gln189

Catalog #FLT-MM23L

Size

100UG

500UG

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Mouse FLT3 Ligand Protein (FLT-MM23L) Summary

Product Description	Recombinant Mouse FLT3 Ligand Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly27-Gln189.
Product Background	Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Mouse FLT3 Ligand Protein (FLT-MM23L) Specifications/Details

Protein	FLT3 Ligand
Synonyms	FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine; Flt-3 Ligand; Flt-3L
Accession	P49772-1
Species	Mouse
Biotinylated	no
Amino Acid Range	Gly27-Gln189
Molecular Weight	The protein has a predicted MW of 45.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test). The affinity constant of 13.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse FLT3 Ligand, hFc Tag captured on CM5 Chip via Protein A can bind Human FLT3, His Tag with an affinity constant of 13.81 nM as determined in SPR assay (Biacore T200).
	Immobilized Human FLT3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse FLT3 Ligand, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA (QC Test).
	Mouse FLT3 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse FLT3 Ligand is greater than 95% as determined by SEC-HPLC.