Mouse Nectin-2/CD112 Protein (NEC-MM102) Summary
Product Description |
Recombinant Mouse Nectin-2/CD112 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Gly351. |
Product Background |
Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Mouse Nectin-2/CD112 Protein (NEC-MM102) Specifications/Details
Protein |
Nectin-2/CD112 |
Synonyms |
CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gln32-Gly351 |
Molecular Weight |
The protein has a predicted MW of 35.7 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse PVRIG, hFc Tag with the EC50 of 0.62ug/ml determined by ELISA (QC Test). The affinity constant of 0.355 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Mouse PVRIG, hFc Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 0.355 uM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse PVRIG, hFc Tag with the EC50 of 0.62ug/ml determined by ELISA (QC Test). |
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Mouse Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse Nectin-2 is greater than 95% as determined by SEC-HPLC. |
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