Name: Non-biotinylated SARS-COV-2 Spike S Trimer (D614G) Protein (COV-VM4LG)
Brand: KactusBio
SKU: COV-VM4LG-100UG
Price: 330.0 USD
Availability: InStock

ELISA binding curve of SARS-CoV-2 Spike S Trimer (D614G) protein analyzed with human ACE2 receptor, showing absorbance at OD450.

Non-biotinylated SARS-COV-2 Spike S Trimer (D614G) Protein (COV-VM4LG)

Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.

SARS-COV-2 Spike S Trimer (D614G) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of SARS-COV-2 Spike S Trimer (D614G) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
Amino Acid Range: Val16-Glu1188(D614G)

Catalog #COV-VM4LG

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Non-biotinylated SARS-COV-2 Spike S Trimer (D614G) Protein (COV-VM4LG) Summary

Product Description	Recombinant SARS-COV-2 Spike S Trimer(D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Glu1188 (D614G) trimer design.
Product Background	The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Non-biotinylated SARS-COV-2 Spike S Trimer (D614G) Protein (COV-VM4LG) Specifications/Details

Protein	Spike S
Synonyms	S protein; Spike glycoprotein; S glycoprotein; COVID-19
Accession	QHD43416.1
Species	SARS-COV-2
Biotinylated	no
Amino Acid Range	Val16-Glu1188(D614G)
Molecular Weight	The protein has a predicted MW of 136.7 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Bis-Tris PAGE result.
Activity	Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
	SARS-COV-2 Spike S Trimer (D614G) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of SARS-COV-2 Spike S Trimer (D614G) is greater than 95% as determined by SEC-HPLC.