Name: SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein (BAS-VM145)
Brand: KactusBio
SKU: BAS-VM145-100UG
Price: 363.0 USD
Availability: InStock

SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein (BAS-VM145)

Immobilized SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.

SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
Amino Acid Range: Gln14-Arg683(T19I, L24S, PPA25-27del, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H)

Catalog #BAS-VM145

Size

100UG

500UG

Order by 4pm ET for delivery on:

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SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein (BAS-VM145) Summary

Product Description	Recombinant SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(T19I, L24S, PPA25-27del, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H).
Product Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein (BAS-VM145) Specifications/Details

Protein	Spike S1
Synonyms	S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Accession	QHD43416.1
Species	SARS-COV-2
Biotinylated	no
Amino Acid Range	Gln14-Arg683(T19I, L24S, PPA25-27del, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H)
Molecular Weight	The protein has a predicted MW of 75.76 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
Activity	Immobilized SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2), His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
	SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) is greater than 95% as determined by SEC-HPLC.